Electrochemical Investigation of Post-Translational Modification-Modulated Biomolecular Liquid-Liquid Phase Separation.

ABSTRACT: Post-translational modification (PTM) plays a key role in the regulation of liquid-liquid phase separation (LLPS), which participates in cell behaviors and pathological processes. Quantitative analysis of PTM-regulated LLPS is therefore essential for understanding cellular processes and discovering new disease targets. However, the membrane-free structure, dynamic characteristics, and distinct chemical microenvironment of biomolecular condensates pose challenges to traditional methods. In this contribution, an electrochemical method is proposed to analyze PTM-regulated LLPS for the first time by exploring its effect on electrochemical species. By taking dephosphorylation as a model, we demonstrate that the degree of LLPS initiated by dephosphorylation can be quantitatively assessed. Import

SUBMITTER: Han Y 

PROVIDER: S-EPMC12458001 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature