Integrative Spatial Omics for Systems-Level Mapping of Pathological Niches.

ABSTRACT: Spatial 'omics technologies are a powerful tool for mapping the relationship between cellular organization and molecular distributions in healthy and diseased tissue microenvironments. Here, we describe a novel multimodal pipeline that represents experimental and computational advances for spatiomolecular analysis of tissue samples across molecular classes. This adaptable method integrates matrix-assisted laser desorption/ionization imaging mass spectrometry spatial lipidomics, spatial transcriptomics, protein imaging via multiplexed immunofluorescence microscopy, and histopathological staining to uncover spatiomolecular profiles associated with unique cellular niches and pathological features. We demonstrate the power of this approach using two different complex human disease systems: Alz

SUBMITTER: Kruse ARS 

PROVIDER: S-EPMC12458195 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature