Patch-Clamp Single-Cell Proteomics in Acute Brain Slices: A Framework for Recording, Retrieval, and Interpretation.

ABSTRACT: Single-cell proteomics (SCP) is a powerful method for interrogating the molecular composition of neurons, yet its application to acute brain slices has remained limited. Patch-clamp electrophysiology provides direct information on neuronal excitability, synaptic inputs, and ion channel function, making it a natural partner for SCP. However, combining these techniques introduces unique challenges: neurons must be both physiologically characterized and physically collected, and variability during retrieval from the brain slice can affect how faithfully proteomic measurements reflect in situ physiology. Here, we introduce a framework for interpreting patch-SCP outcomes that considers retrieval quality in terms of both the amount of material collected and the synaptic contents being rec

SUBMITTER: Rodriguez L 

PROVIDER: S-EPMC12458218 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature