Interdomain disulfide bonds of rabbit kappa light chain allotypes influence mouse-rabbit chimeric antibody performance.

ABSTRACT: Chimeric monoclonal antibodies have emerged as powerful tools to extend the capabilities of traditional monoclonal antibodies. These antibodies are made by replacing the variable region of a monoclonal antibody with the variable region of another antibody, typically from a different species, enabling use in a wider range of applications. Although theoretically compatible, interspecies differences in antibody structure can complicate chimeric antibody design and performance. In this study, we evaluated the impact of rabbit light chain allotype on the expression and function of chimeric antibodies containing mouse light chain variable regions. We found that constructs using the rabbit kappa 1 b4 (K1-b4) allotype frequently exhibited poor recombinant expression and, in some cases, lost antige

SUBMITTER: Park YN 

PROVIDER: S-EPMC12458230 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature