<i>ZNF180</i> modulates tumor intrinsic immunotherapy resistance in melanoma through driving plasticity.

ABSTRACT:

Background

Based on our previous study, we have identified ZNF180, a zinc finger protein, as a pro-tumorigenic regulator in primary melanoma and a marker for poor prognosis. Herein, we report that ZNF180-regulated pathway, hence ZNF180-regulome, underlies resistance towards immune checkpoint inhibitions (ICIs).

Methods

To investigate regulatory roles of ZNF180 to confer these immune suppressive phenotypes, we performed ZNF180 knock-down in melanoma cells in vitro with different genetic backgrounds, namely A375 (BRAF-mutant) and SKMEL147 (NRAS-mutant) cells, and performed RNA- and ATAC-sequencing. We performed integrative analysis of RNA- and ATAC-sequencing data with publicly available sequencing data from ICI-treated c

SUBMITTER: Song WM 

PROVIDER: S-EPMC12458250 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature