<i>In vitro</i> and <i>in vivo</i> derived macrophages occupy distinct phenotypic states.

ABSTRACT: Monocyte-derived macrophages (MDMs) are widely used to model human macrophage biology in vitro and standardized polarization conditions were proposed to recapitulate the different macrophage activation states. Although surface markers specific for distinct MDM populations have been identified, a systematic analysis of surface marker expression on these consensus MDM populations has not previously been reported. Here, we use mass cytometry to perform an in-depth characterization of MDM surface profiles and determine how these markers evolve with time. We also compared the phenotypes found in vitro with patient-derived tumor-associated macrophages (TAMs) and found that although MDMs and TAMs shared most markers investigated, the cell-surface signatures markedly differed in term

SUBMITTER: Chevrier S 

PROVIDER: S-EPMC12458269 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature