MAS Cryoprobe Enhances Solid-State NMR Signals of α-Synuclein Fibrils.

ABSTRACT: Solid-state NMR spectroscopy is increasingly applied to structural and dynamics studies across a broad range of chemical, material, and biological systems. Although sensitivity has traditionally been a major limitation, the recently developed MAS cryoprobe has been shown to substantially overcome this challenge. Its ability to enhance the signal-to-noise (S/N) ratio without requiring sample freezing makes it particularly attractive for investigating non-isotropic systems, including soft materials (e.g., hydrogels), semi-solids (e.g., membrane mimetics) and rigid solids (e.g., amyloid fibrils). In this study, we report on the enhanced sensitivity of solid-state NMR experiments on α-synuclein fibrils using a MAS cryoprobe. Nearly an order-of-magnitude improvement in S/N was observed in CPMAS

SUBMITTER: Widanage MCD 

PROVIDER: S-EPMC12458400 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature