Principles of protein abundance regulation across single cells in a mammalian tissue.

ABSTRACT: Protein synthesis and clearance are major regulatory steps of gene expression, but their in vivo regulatory roles across the cells comprising complex tissues remains unexplored. Here, we systematically quantify protein synthesis and clearance across over 4,200 cells from a primary tissue. Through integration with single-cell transcriptomics, we report the first quantitative analysis of how individual cell types regulate their proteomes across the continuum of gene expression. Our analysis quantifies the relative contributions of RNA abundance, translation, and protein clearance to the abundance variation of thousands of proteins. These results reveal a putative organizing principle: The contributions of both translation and protein clearance are linearly dependent on the cell growth rate.

SUBMITTER: Leduc A 

PROVIDER: S-EPMC12458448 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature