EsBAF and INO80C fine-tune subcompartments and differentially regulate enhancer-promoter interactions.

ABSTRACT: The genome is compacted in the nucleus through a hierarchical chromatin organization, ranging from chromosome territories to compartments, topologically associating domains (TADs), and individual nucleosomes. Nucleosome remodeling complexes hydrolyze ATP to translocate DNA and thereby mobilize histone proteins. While nucleosome remodeling complexes have been extensively studied for their roles in regulating nucleosome positioning and accessibility, their contributions to higher-order chromatin architecture remain less well understood. Here, we investigate the roles of two key nucleosome remodelers, esBAF and INO80C, in shaping 3D genome organization in mouse embryonic stem cells. Using Hi-C, we find that loss of either remodeler has minimal effects on global compartment or TAD structures.

SUBMITTER: Bonilla B 

PROVIDER: S-EPMC12458468 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature