An Indicator Cell Assay-based Multivariate Blood Test for Early Detection of Alzheimer's Disease.

ABSTRACT: The indicator cell assay platform (iCAP) is a novel next-generation approach for blood-based diagnostics that uses standardized cells as biosensors to amplify weak disease signals in blood. We developed an Alzheimer's disease iCAP (AD-iCAP) for early detection at the mild cognitive impairment/mild dementia stages. To develop the assay, patient plasma is incubated with standardized neurons, which transduce complex circulating signals into gene-expression readouts used to train multivariate disease classifiers via machine learning. We applied systems biology analyses (e.g., GSEA, PCA, correlation/network analyses) to optimize analytical and computational parameters, and then evaluated a locked model in a study with retrospectively collected samples. Performance was AUC 0.64 (95% CI 0.51-0.78

SUBMITTER: Qi YS 

PROVIDER: S-EPMC12458623 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature