LTO1 and YAE1 regulate MHC-I expression via nonsense-mediated RNA decay in tumor cells.

ABSTRACT:

Background

Nonsense-mediated messenger RNA decay (NMD) is a highly conserved surveillance system that degrades mRNAs with premature termination codons (PTCs), and regulates the mRNA quantity. Cancer cells hijack NMD to compensate for the imbalanced DNA levels and modulate their antigenicity. We identified the LTO1/YAE1 complex as NMD factors regulating ribosome biogenesis. Our study aimed to investigate their roles in modulating NMD and major histocompatibility complex class I (MHC-I) antigen presentation, which is vital for cancer immunotherapy.

Methods

Clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-associated protein 9 (Cas9)-mediated gene knockouts, overexpression, and mutational analysis were used to explore the role of LTO1 and YAE1 in

SUBMITTER: Yang Z 

PROVIDER: S-EPMC12458808 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature