<b>Functional characteristics of membrane vesicles produced by</b> <i><b>Streptococcus mitis</b></i>.

ABSTRACT:

Objective

Mitis group streptococci (MGS) are the predominant oral bacteria that cause bacteremia and infective endocarditis. Although membrane vesicle (MV) secretion has been reported in Streptococcus pneumoniae among MGSs, comprehensive studies using various streptococcal species are limited. We aimed to determine whether MGS species produce MVs and to examine their biological functions.

Materials and methods

MVs were isolated from MGS cultures using density gradient ultracentrifugation. The particle sizes of MVs were measured, and proteins in MVs were identified by liquid-chromatography tandem mass spectrometry analysis. Effects of MVs on host cells and oral pathogenic bacteria were investigated.

Results

MV production was confirmed in Streptococcus mitis</

SUBMITTER: Matsumoto A 

PROVIDER: S-EPMC12459155 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature