Assessing tissue-specific gene expression of essential genes from human and mouse.

ABSTRACT: A gene is defined as essential when its functional loss compromises an organism's viability. Identifying essential genes is critical for identifying the components that regulate a biological system. Advances in gene editing techniques like CRISPR-Cas9 provide a capacity to interrogate a genome to elucidate the genes that are essential. However, these techniques are often applied for a single-cell line and rarely probed at a level of a tissue or organ. The recent availability of large-scale single-cell RNA-sequencing (scRNA-seq) atlases provides an unprecedented opportunity to investigate essential gene expression in a more comprehensive context. Our study leverages information from benchmarking datasets, single-cell tissue atlases, and databases of essential genes, to develop a method, scE

SUBMITTER: Zheng H 

PROVIDER: S-EPMC12459261 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature