Single-cell RNA sequencing analysis reveals a novel porcine alveolar macrophage subtype with high SLAMF7 expression promoting PRRSV infection.

ABSTRACT: Porcine reproductive and respiratory syndrome virus (PRRSV) is a major and highly contagious swine pathogen that severely impacts the global pork industry. Typically, porcine alveolar macrophages (PAM) isolated from pigs and infected with PRRSV are used to analyze viral pathogenesis, although it remains unclear whether it accurately reflects the in vivo disease process. In this study, scRNA-seq analysis revealed distinct transcriptional profiles between in vitro-cultured and freshly isolated PAMs. Inflammation, apoptosis, autophagy, and the TNF signal pathway were activated both in vivo and in vitro, however, only partial enriched GO terms and KEGG pathways were coincidental. Notably, PRRSV genomes were detected in the T and B cells in vivo, indicating a potential effect of PRRSV on T and

SUBMITTER: Hu A 

PROVIDER: S-EPMC12459362 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature