A workflow for semi-automated volume correlative light microscopy and transmission electron tomography.

ABSTRACT: Volume Correlative Light and Electron Microscopy (vCLEM) is a powerful method for assessing the ultrastructure of molecularly defined subcellular domains. A central challenge in vCLEM has been the efficient navigation of Regions of Interest (ROIs) across multimodal and multiscale imaging datasets. We developed two key tools to overcome this challenge. First, we developed a multimodal image registration tool (SegReg) that utilizes segmentation of common objects across modalities and uses a Graphical Processing Unit (GPU) for registration of imaging datasets in two and three dimensions. Secondly, we developed a dedicated image viewer to visualize multimodal image registration in three dimensions (NavROI). Here, we demonstrate the integrated use of SegReg and NavROI to navigate large mouse ti

SUBMITTER: Konishi K 

PROVIDER: S-EPMC12459417 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature