Field-domain rapid-scan EPR at 240GHz for studies of protein functional dynamics at room temperature.

ABSTRACT: We present field-domain rapid-scan (RS) electron paramagnetic resonance (EPR) at 8.6T and 240GHz. To enable this technique, we upgraded a home-built EPR spectrometer with an FPGA-enabled digitizer and real-time processing software. The software leverages the Hilbert transform to recover the in-phase (I) and quadrature (Q) channels, and therefore the raw absorptive and dispersive signals, χ^' and χ^'', from their combined magnitude (I²+Q²). Averaging a magnitude is simpler than real-time coherent averaging and has the added benefit of permitting long-timescale signal averaging (up to at least 2.5×10⁶ scans) because it eliminates the effects of source-receiver phase drift. Our rapid-scan (RS) EPR provides a signal-to-noise ratio that is ap

SUBMITTER: Price BD 

PROVIDER: S-EPMC12459749 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature