Isolation, purification, and phenotypic characterization of virulent Klebsiella pneumoniae phages from environmental samples in Addis Ababa, Ethiopia: A synergistic approach combining spot assay and streak plating.

ABSTRACT:

Background

Multidrug-resistant (MDR) Klebsiella pneumoniae poses a significant global health threat, necessitating alternative therapies like bacteriophage treatment. Traditional phage isolation methods, such as plaque assays, are resource-intensive and thus limit large-scale screening. This study aimed to isolate and purify phages targeting MDR K. pneumoniae using a combined spot-streak plating method and to compare its efficiency with conventional techniques.

Materials and methods

A total of 62 environmental samples were screened against two MDR K. pneumoniae isolates using a combined approach: initial phage detection by spot assays followed by streak plate-based purification for isolating pure phage clones. Traditional plaque assays were also performed for comparison. Iso

SUBMITTER: Abebe AA 

PROVIDER: S-EPMC12459788 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature