Antiproliferative activity of Grewia villosa ethyl acetate extract on cervical cancer HeLa cell line: Mechanistic insights through network pharmacology and functional assays approach.

ABSTRACT: Grewia villosa is a plant native to Kenya, with a traditional history among Ambeere people for treating and managing prostate and breast cancers. Previous scientific studies have demonstrated its anti-inflammatory and antioxidant properties. However, a scientific gap exists on the bioactivity of G. villosa against cervical cancer, particularly on in vitro HeLa cell line model. Additionally, the specific molecular targets and mode of antiproliferative action have not been well elucidated. Therefore, this study sought to investigate the antiproliferative activity, putative targets and mode of action of G. villosa using in vitro cell culture, molecular biology and in silico-based approaches. Antiproliferative analyses were evaluated through MTT assay, cell migration inhibition through in vitr

SUBMITTER: Kamau SW 

PROVIDER: S-EPMC12459836 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature