UPLC/Q-TOF MS-based femoral muscle metabolomic analysis under high-temperature: a proof of concept for postmortem interval estimation.

ABSTRACT:

Introduction

Accurate postmortem interval (PMI) estimation is a major challenge in forensic medicine, especially for the rapidly and highly putrefied bodies in tropical high-temperature areas. Despite numerous studies, there are still no reliable, objective methods to accurately estimate PMI for corpses found in a high-temperature environment.

Methods

In the current research, an ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS)-based metabolomics approach was employed for the analysis of the metabolic profile of rat femoral muscle tissue in a high-temperature environment at different postmortem intervals. Multivariate pattern recognition and pathway analyses were employed for the identification of differential metabolite

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12460302 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature