Proteomics analysis of soluble secreted proteins of <i>Lutzomyia longipalpis</i> LL5 cells transfected with a dsRNA viral mimic: insights into cellular defense and repair signals.

ABSTRACT: Sand flies, which transmit diseases like leishmaniases, bartonellosis, and certain viruses, pose a significant public health threat. Our research focuses on the immune responses of Lutzomyia longipalpis, the primary vector for visceral leishmaniasis in the Americas. We use L. longipalpis LL5 cells as a model to study how sand flies respond to pathogens. These cells exhibit robust immune reactions, producing molecules mainly regulated by the Toll, IMD, Jak-STAT, and RNAi pathways. In previous studies, we detected a non-specific antiviral response in LL5 cells following double-stranded RNAs (dsRNAs) transfection. A previous complete secretome of these cells showed molecules resembling an interferon-like antiviral response when transfected with polyinosinic-polycytidylic acid (p

SUBMITTER: Martins da Silva A 

PROVIDER: S-EPMC12460361 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature