Production and characterization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymers from a pre- fermented hardwood hydrolysate.

ABSTRACT: Economically viable production of poly(3-hydoxybutyrate-co-3-hydroxyvalerate) (PHBV) copolymers remains a challenge. The objective of this work was to produce low-cost PHBV copolymers from lignocellulose-derived mixed sugars without genetic engineering or addition of chemical precursors. A hardwood hydrolysate was first pre-fermented using the facultative anaerobe Propionibacterium acidipropionici, and the resulting propionate-rich effluent was used for subsequent PHBV biosynthesis in Paraburkholderia sacchari or Hydrogenophaga pseudoflava. P. acidipropionici displayed a high tolerance to the hardwood hydrolysate and produced up to 11 g L^-1 propionate (with varying amounts of lactate and acetate) under batch conditions. Propionic acid exerted significant toxicity toward P. sacch

SUBMITTER: Blunt W 

PROVIDER: S-EPMC12460433 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature