Clostridioides difficile evolution in a tertiary German hospital through a retrospective genomic characterization.

ABSTRACT:

Purpose

Clostridioides difficile is a major cause of healthcare-associated infections, contributing to significant morbidity and mortality. This study aimed to investigate the genomic characteristics, antimicrobial resistance (AMR) profiles, and temporal dynamics of C. difficile strains isolated from hospitalized patients in a German tertiary hospital over nearly two decades (1997-2015).

Methods

Whole-genome sequencing was performed on 46 toxigenic C. difficile isolates to determine sequence types (STs) and phylogenetic relationships and these were compared to national surveillance data on C. dificile. AMR profiling was conducted to identify key resistance determinants at genetic level while epsilometer minimum inhibitory concentration (MIC) analyses were used to correlate g

SUBMITTER: Lorenzo-Diaz F 

PROVIDER: S-EPMC12460453 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature