Micro- and nanoplastics reduce the phagocytosis and intracellular killing of E. coli by THP1-Blue™ NFκB monocytes.

ABSTRACT:

Purpose

Micro- and nanoplastic particles occur ubiquitously in the environment and have been detected in various organs in animals and humans. We studied, how micro- and nanoplastic influence phagocytosis and intracellular killing of live bacteria in human monocytes.

Methods

Cells of the human reporter cell line THP1-Blue™ NFκB were pre-treated with different concentrations of micro- and nanoplastic (diameter 1 μm and 100 nm) and then incubated with Escherichia coli DH5α. Phagocytosis and intracellular killing was studied using an antibiotic protection assay. The activation of the NFκB promoter was quantified by measuring the production of alkaline phosphatase. Cytokines were measured by enzyme immunoassay. Cell viability was determined by trypan blue staining and lactate de

SUBMITTER: Edbauer F 

PROVIDER: S-EPMC12460519 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature