Diesel particulate matter-induced proteomic changes in three-dimensional spheroids derived from human primary cells of various tissue origins.

ABSTRACT: This study investigated the effects of diesel particulate matter (DPM) on multiple human organs using 3D spheroids derived from eight human primary cell types. To assess the impact of DPM, we exposed these spheroids to varying concentrations of standardized DPM (Standard Reference Material, SRM 2975) and measured their viability, followed by proteomic analysis using tandem mass tag (TMT) labeling with liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 9,707 proteins were identified, with 128 proteins exhibiting statistically significant changes (P-value < 0.05) in response to DPM exposure, as determined by two-way analysis of variance (ANOVA). Among these, five proteins, including apolipoprotein A-I (APOA1), significantly increased at higher DPM concentrations, while 36

SUBMITTER: Cho YJ 

PROVIDER: S-EPMC12460833 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature