PDGFC facilitates enzalutamide resistance in prostate cancer through activation of the Rap1-MAPK pathway.

ABSTRACT:

Purpose

Prostate cancer (PCa) is a highly heterogeneous malignant tumor within the male genitourinary system, characterized by its invasive and metastatic potential. Although the second-generation androgen receptor (AR) antagonist enzalutamide has shown therapeutic efficacy in PCa patients, enzalutamide resistance (EnzaR) will inevitably develop and the underlying mechanisms are not fully understood.

Methods

Platelet Derived Growth Factor C (PDGFC) expression in PCa cells was measured by qRT‒PCR and Western blot. The effect of PDGFC on PCa was examined both in vitro and in vivo. CCK8, Colony formation, and EdU assays were used to assess the phenotypes of PCa cells. A tumor xenograft model was used to evaluate the impact of PDGFC on PCa in vivo. Luciferase assays and chromati

SUBMITTER: Deng B 

PROVIDER: S-EPMC12460851 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature