Dehydrozaluzanin C inhibits colon cancer cell proliferation, apoptosis and cycle arrest through peroxisome proliferator-activated receptor γ (PPARγ) activation.

ABSTRACT: Dehydrozaluzanin C (DC) is a sesquiterpene lactone isolated from Asteraceae plant Ainsliaea macrocephala. To investigate the antitumor effects of DC and possible molecular mechanisms for treating cancer. The antitumor effect of DC was studied using HT-29 and HCT-116 human colon tumor cell lines and Balb/c nude mice models. The anti-proliferative, proapoptotic effects, and cycle arrest of DC were observed by cell viability, colony formation, apoptosis, and cycle assays. The changes of protein expression level were examined by Western blot analysis. The transcription activity of PPARγ was determined by Luciferase reporter assay. The role of PPARγ activation in the antitumor activity of DC was verified using PPARγ antagonist GW9662 and si-PPARγ HT-29 cells. DC treatment significantly d

SUBMITTER: Li SS 

PROVIDER: S-EPMC12461092 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature