Novel crosslinked recombinant human collagen scaffolds with excellent cytocompatibility and wound healing effect.

ABSTRACT: Objective: This study aimed to investigate the physicochemical properties, cytocompatibility, and tissue repair effects of novel crosslinked recombinant human collagen (rCOL) scaffolds. Methods: Novel rCOL scaffolds were prepared using EDC/NHS crosslinking and lyophilization. The physicochemical properties of the scaffolds, including the porosity, pore size, crosslinking degree, tensile strength, endotoxin levels, and genotoxicity, were determined. Their cytocompatibility was investigated in vitro, and their tissue repair effects were evaluated in a full-thickness skin wound rat model. Results: The crosslinked rCOL scaffolds exhibited a three-dimensional structure with a porosity exceeding 90% and a pore size ranging from 50 to 400 μm. The crosslinking degree of

SUBMITTER: He Y 

PROVIDER: S-EPMC12461248 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature