Multi-omics integration reveals gut microbiota dysbiosis and metabolic alterations of cerebrospinal fluid in children with epilepsy.

ABSTRACT:

Introduction

Epilepsy is a complex neurological disorder with an unclear pathogenesis. Emerging evidence suggests that gut microbiota dysbiosis and cerebrospinal fluid (CSF) metabolic alterations play a critical role in epilepsy progression through the gut-brain axis. This study aimed to characterize microbial and metabolic disturbances in pediatric epilepsy and identify potential diagnostic biomarkers through integrative multi-omics analysis of matched fecal and CSF samples.

Methods

In this study, we conducted 16S rRNA gene sequencing on fecal samples from a total of 50 participants including 17 common epilepsy (CEP) patients, 23 refractory epilepsy (REP) patients, and 10 non-epilepsy (NEP) patients, along with untargeted metabolomic analysis on 24 paired CSF samples from R

SUBMITTER: Li F 

PROVIDER: S-EPMC12461256 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature