DNA Origami-Based Luminescent Biosensors Enabling Smartphone Detection of Nucleic Acid Sequences.

ABSTRACT: DNA origami enables the precise assembly of complex nanostructures, facilitating the visual detection of various molecular targets. Previously, we developed DNA origami-based nanosensors, termed "pliers", which allowed the detection of inorganic and organic single-molecule targets through shape transitions. However, these methods require costly analytical instruments, such as atomic force microscopy and fluorescence detection. To establish a more accessible and quantitative detection approach, we developed a luminescence-based DNA origami biosensor for nucleic acid detection. By redesigning the sensor module, we improved the structural stability and sensitivity. The integration of split luciferase enabled signal amplification proportional to the target RNA concentration, allowing for preci

SUBMITTER: Kido S 

PROVIDER: S-EPMC12461322 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature