Global Proteome Analysis of a Three-Dimensional Human Chondrocyte Cell Culture System Infected with Chikungunya Virus (CHIKV) Reveals Distinct Immune and Inflammatory Signatures.

ABSTRACT: Chikungunya disease (CHIKD) presents as an acute viral fever with severe joint pain, which may progress into a chronic arthritic condition that can last for months or even years. The cell tropism of chikungunya virus (CHIKV) infection is well studied; however, the mechanisms underlying the pathogenesis of chronicity, especially in the joints, remain poorly understood. Primary chondrocytes were isolated from human joint samples, characterized and grown both as monolayers and as 3D spheroids. These cultures were infected with chikungunya virus (CHIKV) at different multiplicity of infections(MOIs), and a temporal analysis of infection in these distinct types of culture systems was conducted. Further, the global proteome was obtained from these two kinds of culture systems upon infection, and

SUBMITTER: Hasan A 

PROVIDER: S-EPMC12461325 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature