Hyaluronic Acid Immersion Enhances Gamma-Ray-Irradiation Cross-Linking of the Fish-Derived Type I Collagen Membrane.

ABSTRACT: Fish-derived type I collagen is widely used in biomedical implants; however, its limited thermal and enzymatic stability necessitates cross-linking for clinical applications. This study investigated gamma-ray-induced cross-linking of collagen as a shape of membranes, with enhancement via polysaccharide-assisted immersion. Hydrated collagen membranes were irradiated at doses ranging from 15 kDa to 35 kGy to assess their dose-dependent effects. Irradiation at 15 kGy enhanced thermal and enzymatic stability, which declined at higher doses. To further enhance stability, the membranes were immersed in phosphate-buffered saline containing chondroitin sulfate (CS) or hyaluronic acid (HA) during irradiation at 25 kGy. HA-treated membranes showed a synergistic enhancement in thermal stability (T

SUBMITTER: Irawan V 

PROVIDER: S-EPMC12461391 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature