Microfluidic Generation of Oxidized Phospholipid Gradients in Supported Lipid Bilayers to Examine Scavenger Receptor Interactions.

ABSTRACT: Reactive oxygen species (ROS) are responsible for the oxidative truncation of polyunsaturated fatty acids (PUFAs). The products of these reactions have been implicated in many diseases such as cancer and atherosclerosis. As increasing attention is directed toward these oxidized phospholipids (oxPLs), higher throughput methods are needed to examine interactions between oxPLs and scavenger receptors in the immune system. Supported lipid bilayers (SLBs) are a reliable model for lipid membrane-protein interaction studies due to their compositional tunability and ease of integration with a variety of sensing methods. Combining SLBs with microfluidic devices can boost experimental throughput. Herein, we develop a microfluidic gradient generator for creating SLBs that allows for simultaneous anal

SUBMITTER: Berger BA 

PROVIDER: S-EPMC12461685 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature