Spatio-temporal regulation of recombinase expression enables efficient autoexcision of selectable marker genes in soybean and maize.

ABSTRACT:

Background

Most plant transformation protocols include the use of a selectable marker gene to enable efficient selection of transformed cells for transgenic regeneration. While the marker gene facilitates the transformation process, it is not needed once transgenic plants have been identified and is removed during commercial development. Autoexcision is a marker removal process, in which a Cre-lox system is used to remove both the selectable marker gene and the Cre gene itself in the T0 generation. One challenge for autoexcision is achieving efficient marker removal while preserving the recovery rate for transgene-positive transformation events. Predictable and robust control of Cre gene expression is essential to maintaining this balance.

Results

In this report, we demonstr

SUBMITTER: O'Brien B 

PROVIDER: S-EPMC12461952 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature