Comprehensive developmental somatic proteome atlas of Haemonchus contortus underpinned by a chromosome-scale genome and deep tandem mass spectrometry.

ABSTRACT:

Background

Haemonchus contortus is a highly pathogenic, blood-feeding nematode that causes widespread disease and significant economic loss in livestock worldwide. Previous proteomic studies were constrained by incomplete genomic resources and limited analytical sensitivity, impeding comprehensive profiling across life stages.

Methods

In this study, we integrated advanced tandem mass spectrometry with a chromosome-scale genome assembly of the Haecon-5 strain to construct the most detailed somatic proteome of H. contortus to date.

Results

We identified and quantified 7002 proteins across five key developmental stages/sexes-i.e. eggs, third-stage larvae (L3s), fourth-stage larvae (L4s), and adult female (Af) and adult male (Am) worms-tripling the number identified in an

SUBMITTER: Wang T 

PROVIDER: S-EPMC12462265 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature