Spatiotemporal microenvironment landscape and malignant epithelial pattern transition in breast ductal carcinoma progression.

ABSTRACT:

Background

Owing to the complexity of TME components and the heterogeneity of cancer cells, the relationship between the niches of TME and prognosis in breast ductal carcinoma remains unknown. The staged characteristics of corresponding cancer cell behaviors are unclear. Our study aims to reveal spatial structures and specific cellular information of TME and cancer cells subgroups during the progression from DCIS to IDC and lymph node metastasis.

Methods

Single-cell sequencing, spatial transcriptomics, bulk RNA sequencing datasets were used to explore the changes in microenvironmental components and transcriptional programs of tumor cells during the progression of breast ductal carcinoma. Immunohistochemistry, multiplex immunofluorescence, flow cytometry cell cycle detection

SUBMITTER: Cheng X 

PROVIDER: S-EPMC12462311 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature