Comprehensive profiling of immune cell infiltration and biomarker identification in intervertebral disc degeneration.

ABSTRACT: Intervertebral disc (IVD) homeostasis and function depend on the immunological privilege status, while immune cell infiltration constitutes a pivotal pathological driver of intervertebral disc degeneration (IDD). Nevertheless, the immune infiltration landscape and associated molecular targets in IDD remain elusive. We therefore integrated bioinformatic analysis of public datasets and single-cell RNA-sequencing analysis (scRNA-seq) data, flow cytometric (FACS) and experimental validation to delineate immune infiltration dynamics and identify therapeutic targets. CIBERSORT deconvolution analysis revealed significant increases in follicular helper T cells (p < 0.05) and M2 macrophages (p < 0.05) in IDD, with M1 macrophages demonstrating an upward trend. WGCNA showed that the blue module was a

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12462346 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature