Detection and characterization of heteroresistance to chloramphenicol in Klebsiella pneumoniae isolates.

ABSTRACT:

Background

Heteroresistance represents a significant pathway through which sensitive bacteria evolve into resistant strains, posing challenges for current clinical laboratory detection methods.

Objectives

This study aimed to investigate the differences in resistance among K. pneumoniae isolates from various sources, assess the prevalence of chloramphenicol heteroresistance (CHR), and explore the potential causes and key genes associated with CHR.

Methods

K. pneumoniae was isolated from 801 samples obtained from various sources, and its susceptibility to antibacterial agents was assessed. The modified Kirby-Bauer disk diffusion method, population analysis profiling (PAP), and bactericidal curve assays were employed to identify heteroresistant bacteria. Additionally, th

SUBMITTER: Kuang Q 

PROVIDER: S-EPMC12462358 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature