Uncovering the prevalence, key biogenesis enzymes, and biological significance of archaeal lipoproteins.

ABSTRACT: Lipid-anchored proteins are integral components of cell surfaces. In bacteria, lipidation of proteins with a conserved lipobox motif ([L/V/I]^-3 [A/S/T/V/I]^-2 [G/A/S]^-1 [C]⁺¹) is catalyzed by prolipoprotein diacylglyceryl transferase (Lgt). Although lipobox-containing proteins, or lipoproteins, are predicted to be abundant in several archaeal species, no archaeal homologs of Lgt have been identified, suggesting distinct lipidation enzymes evolved in archaea to accommodate their unique membrane lipids. Here, we predicted lipoprotein presence for all major archaeal lineages and revealed a high prevalence of lipoproteins across the domain Archaea. Using comparative genomics, we identified a comprehensive set of candidates for archaeal lipoprotein bio

SUBMITTER: Hong Y 

PROVIDER: S-EPMC12462473 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature