A Combined Neuroanatomy, Ex Vivo Imaging, and Immunohistochemistry Defined MRI Mask for the Human Paraventricular Nucleus of the Thalamus.

ABSTRACT: The paraventricular nucleus of the thalamus (PVT) is an evolutionarily conserved midline thalamic structure known to contribute to arousal, interoceptive states, and motivated behaviors. Yet, a consensus anatomical definition of the human PVT across tissue-based and MRI-based approaches remains elusive, thereby limiting reliable translation between its cellular characteristics and in vivo functional connectivity. To address this challenge, we describe a histologically informed PVT segmentation compatible with standard 3 T MR imaging pipelines. We performed postmortem anatomical MRI scans on an intact whole brain and an excised thalamic block, manually segmented the PVT at high resolution using ex vivo calretinin staining and neuroanatomical landmarks, registered the resulting image-label p

SUBMITTER: Tetzlaff MR 

PROVIDER: S-EPMC12462541 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature