A Fluorogenic Hydrazino-Pictet-Spengler Reaction in Live Cells.

ABSTRACT: Lipid peroxidation generates diverse aldehydic lipids associated with oxidative stress and signaling, yet methodologies capable of their detection in live cells are currently lacking. Here, we report a fluorogenic hydrazino-Pictet-Spengler (HIPS) probe for lipidic aldehydes "FLipA-HIPS" that generates bright, wash-free fluorescent reaction products in vitro and in cellulo. FLipA-HIPS is essentially nonfluorescent but reacts with aldehydes to give an environmentally sensitive fluorophore with a higher quantum yield in SDS micelles as compared to aqueous buffer alone. FLipA-HIPS reacts with lipidic aldehydes to give 10- to 25-fold higher fluorescence intensities than reactions containing either short-chain aliphatic or aromatic aldehydes in aqueous buffer. Confocal microscopy shows that live

SUBMITTER: Basran K 

PROVIDER: S-EPMC12462761 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature