Hyperspectral Fluorescence Imaging with a New Polarity-Ultrasensitive Fluorescent Probe.

ABSTRACT: Hyperspectral fluorescence imaging (HSFI) could simultaneously offer morphological visualization and microenvironmental information through fluorescence wavelength-shifting of microenvironmental-sensitive fluorescent probes, yet its advancement is directly hindered by the scarcity of probes with sufficient spectral sensitivity. Herein, this study focuses on the development of an innovative fluorescent probe that exhibits heightened spectral sensitivity to microenvironmental changes and its advanced HSFI application. First, the comprehensive investigation of various donor-π-acceptor molecules uncovers the critical role of π-spacer on the wavelength sensitivity to microenvironment polarity. Based on this insight, a new fluorescent probe Lipi-PS of whose wavelength sensitivity represents the

SUBMITTER: Zhou R 

PROVIDER: S-EPMC12463004 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature