Tracing and Capturing the Epiblast Pluripotency of Sheep Preimplantation Embryos.

ABSTRACT: Capturing different pluripotent state stem cells from epiblast in vitro helps understand embryonic development and provides invaluable cell sources for basic research and regenerative medicine. Sheep are not only one of the most important livestock species in agriculture but also serve as an ideal preclinical model for studying human disease. Single-cell transcriptome analysis of sheep preimplantation embryos from embryonic day (E) 1 to E14 is performed to investigate the pluripotency changes of epiblast and elucidate the pluripotent regulation signaling. By combination of growth factors or inhibitors of JAK/STAT3, FGF, WNT, and TGF-β pathways in the culture medium, sheep formative and primed pluripotent stem cells (sfPSCs and spPSCs) are established respectively. The newly derived PSCs co

SUBMITTER: Zhang J 

PROVIDER: S-EPMC12463017 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature