Gastrula-Premarked Posterior Enhancer Primes Posterior Tissue Development Through Cross-Talk with TGF-β Signaling Pathway.

ABSTRACT: The regulatory mechanisms governing cell fate determination, particularly lineage diversification during mammalian embryonic development, remain poorly understood with in-depth regulatory paradigms yet to be fully elucidated. Here, leveraging the epigenetic landscape of mouse gastrula, p-Enh is identified, an enhancer located within the first intron of Cdx2 and epigenetically pre-marked in the primitive streak region, as a pivotal regulator for posterior tissue development in mouse embryos. Morphological and single-cell transcriptomic analyses confirmed embryonic lethality phenotype with disrupted posterior tissue development trajectories in p-Enh-KO embryos. Molecularly, apart from regulating the neighboring coding-gene Cdx2 in cis, the findings suggest that p-Enh also modulates the globa

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12463030 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature