A circulating microRNA panel enhances the diagnosis of cholangiocarcinoma.

ABSTRACT:

Background and aim

Cholangiocarcinoma (CCA), a malignancy with a high incidence in regions endemic for the liver fluke Opisthorchis viverrini, is frequently characterized by alterations in the p53 tumor-suppressor gene, a process often modulated by microRNAs. Our study aimed to identify circulating miRNAs as potential diagnostic biomarkers for CCA.

Methods

We sequenced small RNAs to identify differentially expressed microRNAs (miRNAs) in two CCA cell lines (one p53-mutant, one p53-wildtype) relative to an immortalized cholangiocyte cell line. Candidate miRNAs exhibiting significant upregulation with a log2 fold change > 4.5 were selected for validation via RT-qPCR in serum samples from patients with CCA. The diagnostic utility of these serum miRNAs was subsequently evaluated

SUBMITTER: Mon AM 

PROVIDER: S-EPMC12463250 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature