A new investigation of nonalcoholic fatty liver disease: Effects of hypoxia on mitochondrial function and lipid droplet autophagy.

ABSTRACT: An expanding body of research has highlighted the intimate connection between nonalcoholic fatty liver disease (NAFLD) and the dysregulation of hepatic lipid droplet autophagy as well as mitochondrial function. Nevertheless, the intricate interplay between lipid droplet autophagy and mitochondrial function under hypoxic conditions remains largely uncharted territory. Constructing NAFLD mouse models at altitudes of 2200 meters and 4500 meters and simultaneously culturing hepatocytes under oxygen concentrations of 21% and 1%, with the addition of oleic acid to induce lipid accumulation. A comprehensive evaluation of the NAFLD mice at different altitudes was conducted, including a combination of NMR, PAS, and Oil Red O staining, immunofluorescence, qPCR, flow cytometry, ELISA, electron micros

SUBMITTER: Tian M 

PROVIDER: S-EPMC12463273 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature