IKZF1 exacerbates the inflammatory response by epigenetically modulating mitochondrial function following acute peritonitis.

ABSTRACT:

Background

Macrophages play pivotal roles in immune homeostasis and host defense against pathogens, yet their excessive activation can lead to tissue damage. Acute peritonitis induced by cecal ligation and puncture (CLP) is associated with dysregulated macrophage-mediated inflammation. IKZF1, a transcription factor, has been implicated in immune regulation, but its role in CLP-induced macrophage activation remains unclear. This study aimed to investigate the molecular mechanism of IKZF1 in regulating inflammatory responses during acute peritonitis.

Method

Using a murine CLP-induced peritonitis model, we analyzed IKZF1 expression in macrophages via RT-qPCR and western blot. Lenalidomide (Len), an IKZF1 inhibitor, was administered to assess its effects on macrophage inflammati

SUBMITTER: Liu G 

PROVIDER: S-EPMC12463629 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature