High-throughput quantitation of acetaldehyde and ethanol in mice using gas chromatography/mass spectrometry positive chemical ionization.

ABSTRACT:

Background

Acetaldehyde, an immediate ethanol metabolite, mediates many ethanol-induced behavioral effects and is both psychoactive and toxic to animals and humans. Monitoring the kinetics of acetaldehyde using rodent models of alcohol misuse is essential for understanding and managing ethanol-associated diseases. However, quantitation of acetaldehyde in biological specimens after alcohol consumption has been challenging due to its high volatility, relatively low concentrations, and strong reactivity toward biochemical molecules. It was necessary to develop and establish an accurate and high-throughput method to quantitate acetaldehyde and ethanol.

Methods

Gas chromatography/mass spectrometry in positive chemical ionization mode coupled with a 111-vial headspace autosampler

SUBMITTER: Lin YH 

PROVIDER: S-EPMC12463767 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature