Integrated single-cell transcriptomics and Mendelian randomization identify neutrophil-associated biomarker genes in colorectal cancer.

ABSTRACT:

Background

Colorectal cancer (CRC) is a prevalent digestive malignancy imposing significant economic burdens worldwide. However, the role and underlying mechanisms of neutrophil infiltration in CRC remain unclear. We therefore integrated single-cell RNA sequencing (scRNA-seq), Mendelian randomization (MR), and colocalization (coloc) analyses to uncover key neutrophil-related genes in CRC.

Methods

Neutrophil marker genes were identified from scRNA-seq data (GSE231559) retrieved from the Gene Expression Omnibus (GEO). Expression quantitative trait locus (eQTL) data were obtained from the eQTLGen Consortium. An independent cohort from the BioBank Japan (BBJ-a-107) was utilized to conduct two-sample MR analyses, establishing causal relationships between differential gene express

SUBMITTER: Yan H 

PROVIDER: S-EPMC12463778 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature