Harnessing the Tet-Off system for effective gene silencing in <i>Magnaporthe oryzae</i>.

ABSTRACT: Rice blast is the most serious fungal disease affecting rice production worldwide. Studying gene function in the rice-blast fungus Magnaporthe oryzae often presents significant challenges, especially if it involves loci that are essential for the fungal growth and/or viability. Researchers expend considerable effort on the knockout process, only to find their attempts end up in vain. To address this, we introduced a transcriptional regulation system based on the Tet-Off system, designed to conditionally and effectively inhibit the expression of target genes using a tetracycline-controlled module in M. oryzae. Using Agrobacterium tumefaciens mediated-transformation, a Tet-Off cassette was first inserted upstream of the target gene. Stable transformants were obtained in

SUBMITTER: Fang Z 

PROVIDER: S-EPMC12463844 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature