Combined analysis of transcriptome and WGCNA reveals the mediating role of JA in the low male fertility of loquat H30-6.

ABSTRACT: The number of seeds is an important factor limiting the palatability and processing efficiency of loquat. Loquat H30-6 has been found to bear fruits with few seeds, which is attributed to its low male fertility. To investigate the molecular mechanism underlying the low male fertility of H30-6, in this study, RNA-seq was performed using pre-meiotic, meiotic and mature anthers from H30-6 and the other two loquat varieties with normal male fertility. The results indicated that a total of 331 differentially expressed genes (DEGs) were identified between H30-6 and the other two loquats and these DEGs were mainly enriched in pathways related to jasmonic acid (JA). WGCNA revealed that module 'green' were highly positively correlated with seed quantity (r = 0.46), suggesting that module 'gr

SUBMITTER: Wang J 

PROVIDER: S-EPMC12463989 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature